Cart

IonFlux Automated Patch Clamp System

Superior fluidics and assay flexibility with plate reader simplicity and affordability for ion channel research and compound profiling

The IonFlux System offers unmatched flexibility, fluidic sophistication and affordability for ion channel research. Integrating microfluidic perfusion with standard well plates produces an  automated electrophysiology system that looks and operates with the simplicity of a benchtop microplate reader.

High performance in a convenient bench-top platform

  • Giga-ohm seal performance or consistent ensemble recording on one platform
  • Automated temperature control - run from 4º to 40º C
  • Scalable - can be integrated with a liquid handler for walk-away automation
  • Fast compound addition supports both voltage- and ligand-gated ion channels


Replace your manual patch clamp rig

  • Affordable - prices starting at the same level as a manual patch clamp rig
  • Eliminates the technical learning curve required for manual patch clamp


Two systems tailored to your workload requirements

IonFlux HT System
Ideal for high throughput screening of ligand- and voltage-gated ion channels
  • 64 parallel amplifiers, 20-cell ensemble
  • Uses 384-well, SBS format IonFlux plates
  • Throughput up to 8000 data points per day
IonFlux 16 System
Ideal for individual labs that have been limited to manual systems until now
  • 16 parallel amplifiers
  • Uses 96-well, SBS format IonFlux plates
  • Throughput of 2,500 data points per day

Principles of Well Plate Microfluidics

Innovative Well Plate Microfluidic™ Technology combines micron-scale fluidic channels with standard SBS plate formats combining a high degree of functionality and assay flexibility with the added convenience of integration with existing laboratory equipment.

Step 1: Add cells and test compounds on the plates

IonFlux Well Plate Microfluidic consumable

Each IonFlux Plate contains an array of experimental patterns with 12 wells: 8 for compounds, 2 for cell trapping and 2 for cell inlet and outlet.

Cells are loaded in the inlet well, buffer is loaded into the trapping wells, and 8 unique compounds or concentrations series are loaded into the remaining wells of the experimental pattern.

 Step 2: Cells are trapped in an ensemble array

IonFlux Well Plate Microfluidic consumable

Cells are pushed through the main flow channel and pulled onto an ensemble of 20 small pipette-like channels. There are two such ensembles in each experimental pattern which get exposed to the same group of 8 compounds. This provides eight data points in duplicate for enhanced data fidelity.

 Step 3: Current is recorded from each ensemble

IonFlux Well Plate Microfluidic consumable

Continuous recording and superior fluidics are ideal for fast-acting ion channels. Current is measured using a discrete patch clamp amplifier for each ensemble, recording a sum of current across all 20 trapped cells resulting in consistent data.

Ligand-gated ion channel assays

With continuous perfusion as standard IonFlux Systems are ideal for the study of ligand-gated ion channel targets.

  • Superior washout - >50x volume exchange per step
  • Exceptional flexibility and synchronous compound additions across the plate
  • Consistent response data due to ensemble recording

NMDA assay, patch clamp system

Continuous perfusion dramatically improves ligand washout enabling challenging assays such as NMDA.

Voltage-gated ion channel assays

IonFlux systems have features that support analysis of even challenging targets such as sodium channels.

  • Superior concentration clamp due to continuous perfusion
  • High success rates due to ensemble recording
  • Compound off-rate measurements
  • Rleak and Rseries compensation available
IonFlux vgic assays Nav1.8 VGIC: Peak currents above 2nA were recorded for 90% of the ensembles tested. This demonstrates the high success rate achieved for a sodium target.

hERG toxicology and safety screening

The IonFlux System is  uniquely suited for hERG compound profiling work.

  • Ability to work with difficult compounds (low solubility / high lipophillicity)
  • Superior concentration clamp continuous perfusion provides
  • High success rates due to ensemble recording
  • Temperature control to record at near physiological temperatures

Temperature dependent effects

Microfluidic design enables temperature dependant effects on ion channels to be studied in the early stages of drug discovery.

  • Superior temperature control, no thermal shifts during solution change
  • Increased consistency of response by maintaining consistent temperatures across different experiments
  • Temperature can be elevated after the seal step, increasing success rates
  • Fast temperature equilibration time of 30-60s

Related Video

Related Collateral

Brochure


Data Sheet


Applications

       Ligand-gated Ion Channels


       Voltage-gated Ion Channels


Related Webinars

Related Publications

Description Part Number
Heating/cooling module 950-0102
IonFlux 16 Plates (8 per pack, 96-well format) 910-0098
IonFlux HT Plates (8 per pack, 384-well format) 910-0099
IonFlux 16 Giga-ohm/Single Cell Plates (8 per pack, 96-well format) 910-0100
IonFlux HT Giga-ohm/Single Cell Plates (8 per pack, 384-well format) 910-0101