ScanLater Western Blot Assay Kit

Pre-optimized for the ScanLater™ Western Blot Detection Cartridge, this assay kit enables the first western blot detection capability in a microplate reader. Utilizing this substrate-free assay, researchers can achieve equivalent sensitivity to traditional chemiluminescence and fluorescence-based assays, while consolidating western blot and ELISA applications onto a single reader.

ScanLater Western Blot Assay Kit

The ScanLater™ Western Blot Assay Kit is a time-resolved fluorescence (TRF)-based assay optimal for quantitating as little as femtogram protein samples. Using this novel western blot method, researchers can:

Time-resolved fluorescence detection
Europium-labeled goat anti-rabbit, goat anti-mouse, donkey anti-rat, donkey anti-goat or streptavidin labeled antibodies
Supports nitrocellulose and PVDF membranes
340/80 nm excitation with 616/10 nm emission range
Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers
Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate Reader
Eliminate time-dependent substrate addition steps
Sustain blot signal stability for at least one month
Enhance assay sensitivity using TRF method to reduce background noise
Maintain femtogram to picogram protein sensitivity similar to traditional western blot detector

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scanlater-eu-streptavidin-kit, scanlater-anti-mouse-kit, scanlater-anti-rabbit-kit, scanlater-anti-goat-kit, scanlater-anti-rat-kit, scanlater-western-blot-protein-ladder, scanlater-western-blot-assay-kit-components

#R8200

Europium-labeled streptavidin (20 μg)
Blocking buffer (40 mL)
Wash buffer (100 mL)
For 10 mini gels with recommended protocol

#R8203

Europium-labeled streptavidin (60 μg)
Blocking buffer (120 mL)
Wash buffer (300 mL)
For 30 mini gels with recommended protocol

#R8201

Europium-labeled goat anti-mouse antibody (20 μg)
Blocking buffer (40 mL)
Wash buffer (100 mL)
For 10 mini gels with recommended protocol

#R8205

Europium-labeled goat anti-mouse antibody (60 μg)
Blocking buffer (120 mL)
Wash buffer (300 mL)
For 30 mini gels with recommended protocol

#R8202

Europium-labeled goat anti-rabbit antibody (20 μg)
Blocking buffer (40 mL)
Wash buffer (100 mL)
For 10 mini gels with recommended protocol

#R8204

Europium-labeled goat anti-rabbit antibody (60 μg)
Blocking buffer (120 mL), Wash buffer (300 mL)
For 30 mini gels with recommended protocol

#R8224

Europium-labeled donkey anti-goat antibody (20 μg)
Blocking buffer (40 mL)
Wash buffer (100 mL)
For 10 mini gels with recommended protocol

#R8227

Europium-labeled donkey anti-goat antibody (60 μg)
Blocking buffer (120 mL)
Wash buffer (300 mL)
For 30 mini gels with recommended protocol

#R8225

Europium-labeled donkey anti-rat antibody (20 μg)
Blocking buffer (40 mL)
Wash buffer (100 mL)
For 10 mini gels with recommended protocol

#R8228

Europium-labeled donkey anti-rat antibody (60 μg)
Blocking buffer (120 mL)
Wash buffer (300 mL)
For 30 mini gels with recommended protocol

#R8220

7 biotinylated recombinant proteins at 10, 20, 40, 60, 80, 100, and 140 kD
3 pre-stained marker proteins at 18, 31, and 70 kD
Ready to use, for 30 mini gels with recommended protocol

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