ScanLater Western Blot Assay Kit
Pre-optimized for the ScanLater™ Western Blot Detection Cartridge, this assay kit enables the first western blot detection capability in a microplate reader. Utilizing this substrate-free assay, researchers can achieve equivalent sensitivity to traditional chemiluminescence and fluorescence-based assays, while consolidating western blot and ELISA applications onto a single reader.
ScanLater Western Blot Assay Kit
The ScanLater™ Western Blot Assay Kit is a time-resolved fluorescence (TRF)-based assay optimal for quantitating as little as femtogram protein samples. Using this novel western blot method, researchers can:
- Time-resolved fluorescence detection
- Europium-labeled goat anti-rabbit, goat anti-mouse, donkey anti-rat, donkey anti-goat or streptavidin labeled antibodies
- Supports nitrocellulose and PVDF membranes
- 340/80 nm excitation with 616/10 nm emission range
- Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers
- Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate Reader
- Eliminate time-dependent substrate addition steps
- Sustain blot signal stability for at least one month
- Enhance assay sensitivity using TRF method to reduce background noise
- Maintain femtogram to picogram protein sensitivity similar to traditional western blot detector