• Actively cooled capacitor-feedback headstage reduces thermal noise for signal-to-noise ratios close to the theoretical limits of physics
• Integrating headstage mode for measuring sub-picoamp currents to enable high-fidelity single-channel recordings from cell membranes and artificial bilayers
• Resistive headstage mode for larger currents and noise levels in whole-cell recordings
• Slim headstage design for easier access to the preparation on a crowded microscope stage
• Series resistance compensation: Prediction helps achieve the desired command level faster by transiently supercharging the membrane potential
• Series resistance compensation: Correction improves the bandwidth of the recording by eliminating the error introduced by the voltage drop across the series resistance
• Dual-speed current clamp optimizes speed and stability through better matching of the clamp algorithm to experimental parameters
• Integrated seal test in voltage- and current-clamp mode for easy monitoring of membrane and cell health parameters
• Dual command potentials add flexibility by processing input signals from two different sources
• Selectable holding command input with two internal range settings and an external input enable convenient control of the output waveform
• Pipette capacitance compensation neutralizes the contribution of the glass pipette and the pipette holder to the capacitance of the circuitry
• Cell capacitance compensation corrects for the capacitance of the cell membrane in whole-cell recordings
• Selectable output gain with 10 settings to scale the output signal to the desired level
• Leak subtraction for correction of leak currents without software-based algorithms
• Sophisticated 4-pole Bessel low-pass filter acts as anti-aliasing filter and can be used to pre-condition the output signal
• Configurable panel meter displays user-selectable parameters in real time for quick monitoring of the experiment
• Zap facilitates patch rupturing by applying a voltage pulse for a user-defined duration
• Two included pipette holders of different barrel lengths accept glass pipettes in most standard dimensions
• User guide written by scientific consultants, with the assistance of Axon Instruments staff, for straightforward start-up and as an in-depth reference

General Specifications

Detail Specifications

Downloadable PDF Datasheet

In combination with a Digidata® 1550 Data Acquisition System and pCLAMP™ Software, the Axopatch 200B Amplifier forms the foundation of a patch clamp setup like those used in many electrophysiology labs around the world. Telegraphing between the software and the amplifier ensure correct scaling and logging of experimental parameters. The Clampex Data Acquisition Module, which is part of the pCLAMP Software, makes optimal use of the many advanced features of the Axopatch 200B Amplifier.

• Basic Ion Channel Research: Ion channels are pore proteins that are involved in a large variety of physiological processes. Basic research in the ion channel field furthers the understanding of ion channel function as well as their roles within cellular pathways and processes. Single-channel research helps with elucidating the mechanisms underlying ion channel function on a single-molecule level. Whole-cell and perforated patch clamp recordings are typically used to investigate the action of modulators on entire populations of ion channels in the membrane of a cell. Learn more.
• Cell Pathway Analysis: Ion channels are involved in many cell pathways and understanding the function of ion channels in response to changes in membrane potential or the presence or absence of other molecules is important in order to understand exactly how ion channels participate in normal and abnormal biological processes such as cell differentiation and migration, disease states, neuronal communications, etc. Learn more.
• Disease Research: Ion channels play a role in many diseases, including hypertension, cardiac arrhythmias, gastrointestinal, immune and neuromuscular disorders, pathological pain and cancer. By understanding the exact role that ion channels play in a particular disease, researchers might be able to find a way to affect the ion channel in such a way as to alter the course of the disease. Learn more.
• Safety Assessment: A critical part of drug discovery involves safety assessments, such as hERG testing, in order to eliminate any compounds with potential safety concerns. Conventional patch clamp has played an important role in collecting evidence for the absence of side effects before submission of a drug candidate to regulatory authorities. Even if automated electrophysiology has shifted safety testing to earlier stages in the drug discovery process, follow-up with conventional patch clamp is an essential step in the workflow. Learn more.

Accessory categories

• Headstages
• Electrode holders, adapters, and holder components
• Model cells

Headstages

Electrode Holders, Adapters, and Holder Components

Model Cells

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