FLIPR Calcium Assay Kits

FLIPR® Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research.

  • Maximizes fluorescent signal from a wide range of cell types by reducing cell wash artifacts
  • Reduces well-to-well variability, improving assay quality (Z' factor) and reliability (CV %) of high-throughput screens

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FLIPR Calcium Assay Kit

Built as the most comprehensive portfolio of calcium reagents, the kits deliver pre-optimized, homogeneous, fluorescence-based formulations to expedite assay development and screening of GPCR and ion channel targets. FLIPR Calcium Assay Kits provide the most comprehensive method for detecting intracellular calcium changes in a simple and reliable homogeneous assay format. Select from six formulations to complement your target of interest.

FLIPR Assay Kits employ a quenching dye to reduce background fluorescence and improve the signal-to-noise ratio. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits.

Key Assay Features and Benefits

FLIPR Calcium 6 and 6-QF Assay Kits

This exclusive fluorophore features the highest quantum yield of any calcium indicator on the market, delivering the greatest signal window available in a calcium assay kit. The substantial increase in signal amplitude helps to rekindle low signal screens; including endogenous, primary or stem cell targets. Additionally, the novel dye is unaffected by organic anion exchange proteins, enabling measurement with minimal to no probenecid present.

When utilized in the quench-free (QF) formulation, the dye is ideal for quench sensitive targets or assays requiring multiplexing of calcium-flux and luminescent assays within one well.

FLIPR Calcium 5 Assay Kit

A novel fluorophore that amplifies the assay signal window while leveraging the superior masking technology used in the FLIPR Calcium 4 Assay Kit to reduce background fluorescence. In addition to the above assays, receptors with low receptor expression and other targets that give a small calcium response are candidates for this kit.

FLIPR Calcium 4 Assay Kit

Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. It is more inert and reduces compound interference. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors.

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Ordering FLIPR® ASSAY COMPONENTS and Options of FLIPR Calcium Assay Kits

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flipr-calcium-assay-components, flipr-calcium-4-5-6-assay-kits, flipr-calcium-6-assay-kit, flipr-calcium-6-qf-assay-kit, flipr-calcium-5-assay-kit, flipr-calcium-4-assay-kit
#R6133
  • (2) vials of Component A*
  • No Component B included
  • Order 10x dilution/assay buffer (Component B) concentrate R7055 separately

* Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 2 plates..

#R8194
  • (3) vials of Component A* for Calcium 6 assays
  • (3) vials of Component A for Calcium 6-QF assays
  • (1) bottle of dilution/assay buffer (Component B)
  • (3) vials of Component C

* Each reagent vial (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 3 Calcium 6 assay plates and 3 Calcium 6-QF assay plates.

#R8190
  • (10) vials of Component A*
  • (1) bottle of dilution/assay buffer (Component B)

* Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 10 plates

#R8191
  • (10) vials of Component A*
  • Order 10x dilution/assay buffer (Component B) concentrate R7055 separately

* Each reagent vial (Component A) is sufficient for 10 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8195
  • (2) vials of Component A*
  • Order 10x dilution/assay buffer (Component B) concentrate R7055 separately

* Each reagent vial (Component A) is sufficient for 50 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R6134
  • (2) vials of Component A*
    • No Component B included
    • Order 10x dilution/assay buffer (Component B) concentrate R7055 separately
  • (2) vials of Component C *

* Each reagent vial (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 2 plates.

#R8192
  • (10) vials of Component A*
  • (1) bottle of dilution/assay buffer (Component B)
  • (10) vials of Component C

* Each reagent vial (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 10 plates.

#R8193
  • (10) vials of Component A*
  • Order 10x dilution/assay buffer (Component B) concentrate R7055 separately
  • (10) vials of Component C

* Each reagent vial (Component A, C) is sufficient for 10 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8196
  • (2) vials of Component A*
  • Order 10x dilution/assay buffer (Component B) concentrate R7055 separately
  • (2) vials of Component C

* Each reagent vial (Component A, C) is sufficient for 50 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8188
  • (2) vials of Component A*
    • No Component B included
    • Order 10x dilution/assay buffer (Component B) concentrate R7055 separately
  • * Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 2 plates.
#R8185
  • (10) vials of Component A*
  • (1) bottle of dilution/assay buffer (Component B)

* Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 10 plates.

#R8186
  • (10) vials of Component A*
  • Order 10x dilution/assay buffer (Component B) concentrate R7055 separately

* Each reagent vial (Component A) is sufficient for 10 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates..

#R8187
  • (2) vials of Component A*
  • Order 10x dilution/assay buffer (Component B) concentrate R7055 separately

* Each reagent vial (Component A) is sufficient for 50 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8142
  • (10) vials of Component A*
  • (1) bottle of dilution/assay buffer (Component B)

* Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 10 plates.

#R8141
  • (10) vials of Component A*
  • Order 10x dilution/assay buffer (Component B) concentrate R7055 separately

* Each reagent vial (Component A) is sufficient for 10 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8143
  • (2) vials of Component A*
  • Order 10x dilution/assay buffer (Component B) (R7055) separately

* Each reagent vial (Component A) is sufficient for 50 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8172
  • (3) vials Component A* for Calcium 4 assays
  • (3) vials Component A* for Calcium 5 assays
  • (3) vials Component A* for Calcium 6 assays
  • (1) bottle of dilution/assay buffer (Component B)

* Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well).

#R7055
  • contains (1) bottle of 10x dilution/assay buffer concentrate
  • each bottle is sufficient for preparing 1000 ml 1x dilution/assay buffer (Component B) to use with FLIPR Calcium Kits (part number R8191, R8195, R8193, R8196, R8186, R8187, R8141, R8143)
  • Fura 2 QBT Calcium Kits (part number R6139, R8198)
  • FLIPR Membrane Potential Kits ( part number R6135, R6136, R6137)
  • FLIPR Potassium Kit (part number R8223)

* This product is free of animal-derived component.

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